Investigations of infected cases in Kirkuk city center through quantitative and qualitative methods of Toxoplasma gondii

Author's Information:

Torken Ahmed Hama Hasan

Department of Biology Tuzkhurmatu Education college. University of Tikrit. Iraq

Vol 02 No 06 (2025):Volume 02 Issue 06 June 2025

Page No.: 192-200

Abstract:

Blood samples were taken from 100 blood donations using private laboratories in Kirkuk city. The IHAT, LAT and ELISA tests were used on their blood plasma to see if they had become infected with the Toxoplasma gondii parasite. Researchers learned that, according to direct hemagglutination, the overall infection rate was 44% and by indirect hemagglutination, the rate reached 60%. According to ELISA, 71% of the samples were infected with the virus. After testing methods, it was observed that 44% of samples became infected with a parasite by direct hemagglutination, while 60% became infected by indirect hemagglutination. The infection was found with a 71% positive result by ELISA. The authors observed that the ELISA approach was the most precise method of all in their study, with indirect hemagglutination coming in second and direct hemagglutination ranking third in detecting antibodies. Similarly, T. gondii infection rates were mainly observed in people younger than 35. Our study revealed that the infection rate in people from cities was (75%), while the rate in rural areas was just (25%). The important factors supporting the spread of the parasite are contact with soil, the level of education and keeping cats. ELISA testing revealed that of the infected cases, 75% showed positivity for the IgG antibody compared to 25% for the IgM antibody.

KeyWords:

Toxoplasma gondii , quantitative , qualitative , Kirkuk city

References:

Yan, C., Liang, L. J., Zheng, K. Y., & Zhu, X. Q. (2016). Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii. Parasites & vectors, 9, 1-7. DOI 10.1186/s13071-016-1432-6

de Noya, B. A., & Ruiz-Guevara, R. (2020). Pregnancy as a risk factor to disease and the vertical transmission to the fetus, of a host of parasitic ailments. 3 https://doi.org/10.47449/CM.2020.1.1.5.

Bhowmik, A. (2023). Role of Diagnostic procedures in managing human Bacterial infections: A comprehensive overview. Archives of Hematology Mini Reviews and Reviews, 8(1), 008-019. 10.17352/ahcrr.000043

Landsman, G. (2003). Emplotting children's lives: Developmental delay vs. disability. Social Science & Medicine, 56(9), 1947-1960. https://doi.org/10.1016/S0277-9536(02)00215-0

Cordeiro, C. N., Tsimis, M., & Burd, I. (2015). Infections and brain development. Obstetrical & gynecological survey, 70(10), 644-655. DOI: 10.1097/OGX.0000000000000236.

Givens, M. D., & Marley, M. S. D. (2008). Infectious causes of embryonic and fetal mortality. Theriogenology, 70(3), 270-285. https://doi.org/10.1016/j.theriogenology.2008.04.018

Ducrocq, J., Simon, A., Lemire, M., De Serres, G., & Lévesque, B. (2021). Exposure to Toxoplasma gondii through consumption of raw or undercooked meat: a systematic review and meta-analysis. Vector-Borne and Zoonotic Diseases, 21(1), 40-49. https://doi.org/10.1089/vbz.2020.2639

Naeem, M. I., Younus, M., Nisa, Q., Akhtar, T., & Kausar, R. Irza and Muzzafar HA, 2023. Zoonotic infertility due to toxoplasma gondii. Zoonosis, Unique Scientific Publishers, Faisalabad, Pakistan, 2, 412-422.https://doi.org/10.47278/book.zoon/2023.79

Singh, S., & Munawwar, A. (2010). Human toxoplasmosis: A food borne Parasitic Disease. PROF. SARMAN SINGH, 15.

Taghipour, A., Ghodsian, S., Jabbari, M., Olfatifar, M., Abdoli, A., & Ghaffarifar, F. (2021). Global prevalence of intestinal parasitic infections and associated risk factors in pregnant women: a systematic review and meta-analysis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 115(5), 457-470.

Shang, Y. U., Tang, L. H., Zhou, S. S., Chen, Y. D., Yang, Y. C., & Lin, S. X. (2010). Stunting and soil-transmitted-helminth infections among school-age pupils in rural areas of southern China. Parasites & vectors, 3, 1-6.

Mutebi, F., Krücken, J., von Samson-Himmelstjerna, G., Waiswa, C., Mencke, N., Eneku, W., & Feldmeier, H. (2018). Animal and human tungiasis-related knowledge and treatment practices among animal keeping households in Bugiri District, South-Eastern Uganda. Acta tropica, 177, 81-88.

Chernecky, C. C., & Berger, B. J. (2012). Laboratory tests and diagnostic procedures. Elsevier Health Sciences.

Bhat, A. I., Rao, G. P., Bhat, A. I., & Rao, G. P. (2020). Serological Tests.

Cork, S. C., Careem, M. F. A., & Abdul-Cader, M. S. (2019). Serology and immunology. The Veterinary Laboratory and Field Manual 3rd Edition, 6.

Hussien, M. R., Al-Saeed, A. T., & Eassa, S. H. (2018). Toxoplasmosis seropositivity and male sex hormones. J. Immunol Infect Dis, 5(1), 1-8.

Al-Adlan, A. A. J. (2007). Diagnostic and Serological Study of Toxoplasma gondii in Aborted Women Using PCR Technique in Dhi Qar Governorate (Doctoral dissertation, MS thesis, Coll. Educ., Dhi Qar Univ., Iraq).

Ashraf, H., Beltinger, J., Alam, N. H., Bardhan, P. K., Faruque, A. S. G., Akter, J., ... & Gyr, N. (2007). Evaluation of faecal occult blood test and lactoferrin latex agglutination test in screening hospitalized patients for diagnosing inflammatory and non-inflammatory diarrhoea in Dhaka, Bangladesh. Digestion, 76(3-4), 256-261.

Ahmed, F., Cappai, M. G., Morrone, S., Cavallo, L., Berlinguer, F., Dessì, G., ... & Varcasia, A. (2021). Raw meat based diet (RMBD) for household pets as potential door opener to parasitic load of domestic and urban environment. Revival of understated zoonotic hazards? A review. One Health, 13, 100327.

Ibrahim, H. M., Huang, P., Salem, T. A., Talaat, R. M., Nasr, M. I., Xuan, X., & Nishikawa, Y. (2009). Prevalence of Neospora caninum and Toxoplasma gondii antibodies in Northern Egypt. American Journal of Tropical Medicine and Hygiene, 80(2), 263-267.

Noorbakhsh, S., Mamishi, S., Rimaz, S., & Monavari, M. R. (2002). Toxoplasmosis in primiparus pregnant women and their neonates.

Mabaso, M., Sokhela, Z., Mohlabane, N., Chibi, B., Zuma, K., & Simbayi, L. (2018). Determinants of HIV infection among adolescent girls and young women aged 15–24 years in South Africa: a 2012 population-based national household survey. BMC public health, 18, 1-7.

High, K. P. (2004). Infection as a cause of age-related morbidity and mortality. Ageing Research Reviews, 3(1), 1-14.

Felizardo, A. A., Marques, D. V. B., Caldas, I. S., Goncalves, R. V., & Novaes, R. D. (2018). Could age and aging change the host response to systemic parasitic infections? A systematic review of preclinical evidence. Experimental gerontology, 104, 17-27.

Izhar, R., & Ben‐Ami, F. (2015). Host age modulates parasite infectivity, virulence and reproduction. Journal of Animal Ecology, 84(4), 1018-1028.

Rasheed, P., & Al-Sowielem, L. S. (2003). Health education needs for pregnancy: a study among women attending primary health centers. Journal of Family and Community Medicine, 10(1), 31-38.

Darnton-Hill, I., & Mkparu, U. C. (2015). Micronutrients in pregnancy in low-and middle-income countries. Nutrients, 7(3), 1744-1768.Yang, W. N. (2016). PARASITES. Physical and Biological Hazards of the Workplace, 471-499.

Overgaauw, P. A. (2020). Parasite risks from raw meat-based diets for companion animals. Companion Animal, 25(11), 261-267.

Englar, R. E. (2019). Common clinical presentations in dogs and cats. John Wiley & Sons.

Jumaian, N. F. (2005). Seroprevalence and risk factors for Toxoplasma infection in pregnant women in Jordan. Eastern Mediterranean health journal, 11(1/2), 45.

Berger, C. N., Sodha, S. V., Shaw, R. K., Griffin, P. M., Pink, D., Hand, P., & Frankel, G. (2010). Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental microbiology, 12(9), 2385-2397.

Dixit, B., Meshram, S., Jha, A. K., & Khare, R. (2024). Parasitic Fauna Associated with Reproductive Disorders. Principles and Practices of Canine and Feline Clinical Parasitic Diseases, 161-172.

Board, R. G., & Tranter, H. S. (2017). The microbiology of eggs. In Egg science and technology (pp. 81-104). CRC Press.

Yan, C., Liang, L. J., Zheng, K. Y., & Zhu, X. Q. (2016). Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii. Parasites & vectors, 9, 1-7.

Sandee, T. (2016). Intestinal parasites and hookworm species in stray cats, dogs and soil: An epidemiological approach to study soil contamination with zoonotic parasites/Sandee Tun (Doctoral dissertation, University of Malaya).

Tun, S. (2016). Intestinal Parasites and Hookworm Species in Stray Cats, Dogs and Soil: An Epidemiological Approach to Study Soil Contamination with Zoonotic Parasites. University of Malaya (Malaysia).

Zhang, X. J., Shen, Q., Wang, G. Y., Yu, Y. L., Sun, Y. H., Yu, G. B., ... & Ye, D. Q. (2009). Risk factors for reproductive tract infections among married women in rural areas of Anhui Province, China. European Journal of Obstetrics & Gynecology and Reproductive Biology, 147(2), 187-191.

Bateman, P. W., & Fleming, P. A. (2012). Big city life: carnivores in urban environments. Journal of zoology, 287(1), 1-23.

Yan, C., Liang, L. J., Zheng, K. Y., & Zhu, X. Q. (2016). Impact of environmental factors on the emergence, transmission and distribution of Toxoplasma gondii. Parasites & vectors, 9, 1-7.

Zhu, S., VanWormer, E., & Shapiro, K. (2023). More people, more cats, more parasites: Human population density and temperature variation predict prevalence of Toxoplasma gondii oocyst shedding in free-ranging domestic and wild felids. PLoS One, 18(6), e0286808.

Mahmood, S. I., Saeed, N. A. H. A. A., & Ali, L. Q. (2022). Rubella Virus and Toxoplasma Gondii Infection With Immune Antibodies Diagnosis Among Bad Obestetric And Primigravida Pregnant Women In Baghdad CITY. World Bulletin of Public Health, 12, 78-85.

Luo, C., Liu, M., Li, Q., Zheng, X., Ai, W., Gong, F., ... & Luo, J. (2021). Dynamic changes and prevalence of SARS-CoV-2 IgG/IgM antibodies: Analysis of multiple factors. International Journal of Infectious Diseases, 108, 57-62.

Dailey, A. F. (2017). Vital signs: human immunodeficiency virus testing and diagnosis delays—United States. MMWR. Morbidity and mortality weekly report, 66.